scrambled negative control sgrna cat Search Results


86
Danaher Inc dsirna
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Qiagen scramble-ish custom lna detection probe lncrna and mrna cat. no: 3395085-3dig lcd0000002bdg
Scramble Ish Custom Lna Detection Probe Lncrna And Mrna Cat. No: 3395085 3dig Lcd0000002bdg, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene scrambled negative control sirna origene cat. n° sr30004
Scrambled Negative Control Sirna Origene Cat. N° Sr30004, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen allstars negative control sirna
Allstars Negative Control Sirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene scramble control
Scramble Control, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher sequence sirna
Sequence Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher non targeting sirna
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Non Targeting Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology universal negative control sirna duplex scrambled
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Universal Negative Control Sirna Duplex Scrambled, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene trilencer-27 universal scrambled negative control sirna duplex
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Trilencer 27 Universal Scrambled Negative Control Sirna Duplex, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher pre-scrambled mirna control nc
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Pre Scrambled Mirna Control Nc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology control sirna 37007
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Control Sirna 37007, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Qiagen non-specific control sequence
HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using <t>siRNA</t> in ESHF derived <t>CDCs</t> <t>(ESHF(HSF1-KD)).</t> (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.
Non Specific Control Sequence, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using siRNA in ESHF derived CDCs (ESHF(HSF1-KD)). (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.

Journal: Stem cells (Dayton, Ohio)

Article Title: Cardiosphere Derived Cells from Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity due to the Heat Shock Response Regulating the Secretome

doi: 10.1002/stem.1937

Figure Lengend Snippet: HSR plays a vital role in the functional potential of hCDCs. HSF-1 transcription factor was knock down (KD) using siRNA in ESHF derived CDCs (ESHF(HSF1-KD)). (A) Knockdown of HSF1 by siRNA, administration, and sample collection was performed as outlined. (B–C) HSF-1 and HSP70 expression was decreased to less than 5% expressed in ESHF (HSF1-KD). (D) HSF-1 KD reduced the expression of HSF-1 and VEGF-1A protein. (E–I) Secretion of paracrine factors was significantly reduced after HSF-1 knockdown in ESHF derived CDCs: VEGF-1A (P=0.03), SDF-1α (P=0.0043), PGDFB (P=0.008), ANG-2 (P=0.016) and FGF-2 (P=0.006) but no difference was seen for HGF. (K) Representative images of HUVEC tube formation (left) and quantification of total tube length (right) after plating HUVEC cells on matrix coated wells with IMDM condition medium from ESHF and ESHF (HSF1-KD) CDCs (scale bar = 100µm) (****=P<0.0001, ***=P<0.001, **=P<0.01). (L) Knockdown of HSF-1 in ESHF derived CDCs resulted in reduction of left ventricle ejection fraction as determined by echocardiography at 7 days and at 28 days.

Article Snippet: The siRNA duplexes for human-HSF1 (Cat # s6950) and scrambled non-targeting siRNA (Silencer select negative control cat # 4390844) were obtained from Ambion.

Techniques: Functional Assay, Derivative Assay, Expressing